United start the home campaign at Barclays Under-21 Premier League level with Wednesday night's fixture against Tottenham.
Warren Joyce's side will look to follow up the success on the road against Stoke City after the Under-18s won their clash with Spurs on Saturday.
"Spurs Under-21s lost their first game 6-4 to Newcastle but we don't know them too well so it's going a little bit into the unknown for us," Joyce told ManUtd.com. "I don't think we've really played against Tottenham before at Reserves level. Not that I can recall over the last few years anyway."
Darren Fletcher captained the youngsters to the opening-day win against the Potters and his professional attitude shone through again as he continues to bid for a return to the first team.
"It was great for 'Fletch' and I thought he did really well in the game," added Joyce. "He did his job and Stoke's older players were in the areas of the field he was operating in so it must've been great for him to come through 90 minutes like that.
"He's a good example for everybody because he treats the games as if they are first-team matches."