The Scottish midfielder has been out of action since the Reds were beaten by Liverpool in early March, but will feature at Old Trafford as he prepares to rejoin the senior squad’s quest for the Premier League and Champions League.
Fletcher will be joined by several players with first team experience: Wes Brown, Gabriel Obertan, Bebe and Ben Amos all start for the Reds, who will be captained by Scott Wootton.
Supporters who wish to attend the match, which may see United clinch top spot in the Reserve League’s Northern Group A, can gain entry at Old Trafford for £5. Entry is free for season ticket holders, members, under-16s and over-65s.
The match will also be televised live on MUTV. Coverage begins at 18:30 BST, 30 minutes before kick-off.
United: Amos, W Brown, Wootton, Gill, Dudgeon; Bebe, R Brown, Norwood, Fletcher, Brady; Obertan.
Subs: Devlin, Vermijl, Petrucci, Tunnicliffe, W Keane.