Sam Johnstone, Michael Keane and Tom Thorpe will make the trip to Estonia and gain some valuable experience of tournament football.
Johnstone captained the team in the qualifiers while Michael Keane impressed after making his England debut last month. FA Youth Cup-winning skipper Thorpe keeps his place after initially being drafted in by coach Noel Blake as cover for the Elite Round.
Will Keane misses out on certain inclusion after suffering cruciate knee ligament damage in the qualifier against Switzerland while Larnell Cole is another absentee following his injury at the back-end of the campaign.
Full squad: Sam Johnstone (United), Connor Ripley (Middlesbrough); Eric Dier (Sporting Lisbon), Luke Garbutt (Everton), Michael Keane (United), Jack Robinson (Liverpool), Tom Thorpe (United); Conor Coady (Liverpool), Ross Barkley (Everton), Nathaniel Chalobah (Chelsea), Harry Kane (Tottenham), John Lundstram (Everton), Nathan Redmond (Birmingham), George Thorne (West Brom); Benik Afobe (Arsenal), Saido Berahino (West Brom), Robert Hall (West Ham), Hallam Hope (Everton).