The United manager has the opportunity to claim his first piece of silverware on Sunday with victory over the Championship side, now managed by Moyes' fellow Scot and friend Owen Coyle.
After confirming exclusively to MUTV that Nani, Ashley Young and Wayne Rooney are not fit for the match at Wembley, Moyes talked about the importance of the game to the club, particularly with a trophy up for grabs.
"It’s a really important fixture because it’s the start of the season for us. Obviously the Premier League season starts next weekend but this is the first real serious game of note, albeit it is still in pre-season, but there is a trophy at stake," he told MUTV.
"I think Wigan will be one of the sides pushing to get back in the Premier League again. They had a great start in their first Championship match against Barnsley so it will be a tough game. They won last time they went to Wembley so we have got to make sure we do a good job and stop them this time.
"This football club is used to winning trophies and we will do everything we can to make sure we win another."
Moyes was also asked about the situation regarding Wayne Rooney's fitness after the striker was selected for the England squad for their international with Scotland in midweek.
"I am pleased about that," said the United boss. "I had a long conversation with Roy [Hodgson, England manager] last week about it and told him Wayne’s situation.