Lingard and Zaha are ineligible for the Toulon tournament in the summer as they are too old for the Under-20 event. England face Qatar in their opening fixture on 22 May with Johnstone and Keane in the coach's plans.
"It’s only the 1992 birthdays like Jesse who couldn’t come to that and Wilf is the same," added Southgate. "But lots of the others are eligible. We need to expose the boys to a different sort of challenge and will play teams from South America and Asia within that. It’s just a different style and we’ll find out whether we can defend and how we need to defend against that sort of opposition."