The Brazilian midfielder has been out of action since suffering a badly-gashed leg in September's Carling Cup win over Middlesbrough, but will line up against Manchester City at Ewen Fields.
Possebon is joined by Rafael, Manucho, Darron Gibson and goalkeeper Ben Foster in bringing first team experience to a strong Reserves side.
Foster presides over a defence comprising Rafael, Richard Eckerlsey, David Gray and Corry Evans, while Possebon and Gibson join Tom Cleverley, Sam Hewson and Danny Drinkwater in a fluid midfield.
Manucho will start as the Reds' lone striker, while Ole Gunnar Solskjaer and Warren Joyce have plumped for a bench featuring Ron-Robert Zieler, Jimmy Derbyshire, Magnus Eikrem, Matt James and Anton Bryan.
The match will be shown live on MUTV, with coverage starting at 18:30 BST.