The Moss Lane fixture, a second outing at Altrincham's ground this season, could allow Warren Joyce the chance to pit his youngsters against experienced opponents as the Reds look to defend the trophy obtained following the victory over Manchester City in August's final.
Bolton defeated Blackpool in the first round of the competition while Burnley and Wigan Athletic have already booked their places in the last four.
The Reds have already triumphed 4-0 against Wanderers in the Manchester Senior Cup back in September but only Ben Amos, Guillermo Varela, Charni Ekangamene and Reece James from the players who got on the pitch that night are likely to be available. Ekangamene is on loan at Carlisle but featured at centre-back in the Under-21s' win at Wolves last time out.
Striker James Wilson, who scored all three goals at Molineux, will be a major threat if he is involved again.