Edwin van der Sar, Ryan Giggs and Patrice Evra all appeared to come through training unscathed as United spent an hour on the Stadio Olimpico pitch on Monday night.
Michael Carrick, however, was forced to watch from the sidelines after picking up an injury in the Reds’ weekend win over Aston Villa.
Earlier on Monday, Sir Alex Ferguson admitted there was some uncertainty surrounding Giggs and Evra’s fitness, but neither showed any sign of discomfort during the evening session.
The Reds began with light stretching before progressing to some basic ball work and a small-sided game.
Mikael Silvestre, with only two Reserves games under his belt since damaging cruciate ligaments last September, looked fit and sharp.
Rio Ferdinand, who also came off against Villa, looked fine in the session, despite nursing a black eye sustained during Saturday’s league win.