The Reserves' home games will take place at Moss Lane for the remainder of the 2008/09 season.
The Reds have spent the last 14 months playing at the Marston's Arena in Northwich, but have now switched to the home of Altrincham FC.
The switch marks a return to Moss Lane for the Reds, who used the ground between 2002 and 2005, prior to using Hyde United's Ewen Fields.
Last week's Manchester Senior Cup defeat to Bolton took place at Moss Lane, and the Reds will next be in competitive action against Middlesbrough on 27 November.
Prices for all home games will remain at £5 for adults and £2 for concessions, while season ticket holders and One United members can gain free entry.